Two types of Chlamydomonas flagellar mutants missing different components of inner-arm dynein.
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چکیده
منابع مشابه
Two types of Chlamydomonas flagellar mutants missing different components of inner-arm dynein
Two types of Chlamydomonas reinhardtii flagellar mutants (idaA and idaB) lacking partial components of the inner-arm dynein were isolated by screening mutations that produce paralyzed phenotypes when present in a mutant missing outer-arm dynein. Of the currently identified three inner-arm subspecies I1, I2, and I3, each containing two heterologous heavy chains (Piperno, G., Z. Ramanis, E. F. Sm...
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In the mechanism underlying the phototactic behavior of Chlamydomonas, Ca(2+) has been thought to control the dominance between the two flagella so as to steer the cell to correct directions. A newly isolated mutant, lsp1, that displays weak phototaxis was found to be defective in this Ca(2+)-dependent shift in flagellar dominance; in demembranated and reactivated cell models, the trans flagell...
متن کاملFunctional recombination of outer dynein arms with outer arm-missing flagellar axonemes of a Chlamydomonas mutant
A flagellar mutant of Chlamydomonas, oda, lacks the entire outer dynein arm but can swim at a speed of one third to half of that of the wild type. We found that the addition of a high-salt extract of wild-type axonemes to demembranated oda cell models restored up to 83% of the outer arms normally present on the outer-doublet microtubules of wild-type axonemes. Furthermore, when reactivated in t...
متن کاملA tektin homologue is decreased in chlamydomonas mutants lacking an axonemal inner-arm dynein.
In ciliary and flagellar axonemes, various discrete structures such as inner and outer dynein arms are regularly arranged on the outer doublet microtubules. Little is known about the basis for their regular arrangement. In this study, proteins involved in the attachment of inner-arm dyneins were searched by a microtubule overlay assay on Chlamydomonas mutant axonemes. A 58-kDa protein (p58) was...
متن کاملRegulation of Flagellar Dynein by Phosphorylation of a 138-kD Inner Arm Dynein Intermediate Chain
One of the challenges in understanding ciliary and flagellar motility is determining the mechanisms that locally regulate dynein-driven microtubule sliding. Our recent studies demonstrated that microtubule sliding, in Chlamydomonas flagella, is regulated by phosphorylation. However, the regulatory proteins remain unknown. Here we identify the 138-kD intermediate chain of inner arm dynein I1 as ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1991
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.112.3.441